Standard Approach (3 mL = ~6.67 mg/mL)
| Days |
Example amount |
Units (per measurement) (mL) |
| Days 1–5 |
1.0 mg (1,000 mcg) |
15 units (0.15 mL) |
| Days 6–14 |
1.5 mg (1,500 mcg) |
22.5 units (0.225 mL) |
| Days 15–20 |
2.0 mg (2,000 mcg) |
30 units (0.30 mL) |
Frequency: Measure once daily, preferably in the morning or early afternoon. Standard cycles are 10–20 days, with the option to extend to 28 days if desired[5][6]. Repeat cycles can be prepared every 2–3 months as needed.
Reconstitution Steps
- Draw 3.0 mL bacteriostatic water with a sterile syringe.
- Measure slowly down the vial wall; avoid foaming.
- Gently swirl/roll until dissolved (do not shake).
- Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.
How This Works
Pinealon is a synthetic tripeptide that appears to cross cellular and nuclear membranes to interact directly with DNA, thereby regulating gene expression in brain tissue[4]. Research indicates it reduces reactive oxygen species accumulation and necrotic cell death in neurons while modulating the cell cycle to activate proliferative pathways[3][8]. Preclinical studies demonstrate neuroprotective effects against oxidative stress, ischemic injury, and cognitive decline, with improvements in memory retention and learning capacity observed in animal models[9][10]. Clinical trials in humans with traumatic brain injury and cerebral disorders have reported improvements in memory, attention, cognitive function, and emotional stability with daily preparation of oral or injectable Pinealon[6][11].
References
Biogerontology (Springer)
— Peptide bioregulation of aging: 35‑year research overview by Khavinson and Anisimov
Advances in Gerontology (Springer)
— Peptide bioregulators as geroprotectors: experimental studies review
Rejuvenation Research
— Pinealon increases cell viability by suppressing ROS and activating proliferation
Biotech Peptides Research Review
— Pinealon mechanism of action: direct DNA interaction and gene regulation
LIVV Natural Medical Review
— Pinealon in neurological disorders: clinical applications and protocols
Innerbody Research
— Pinealon peptide: comprehensive review of benefits, safety, and clinical use
Paragon Sports Medicine
— Pinealon peptide: brain health, memory, and cognitive enhancement research
Core Peptides Research
— Pinealon effects on neuroprotection, cell vitality, and aging processes
PMC (PubMed Central)
— Pinealon protects rat offspring from prenatal hyperhomocysteinemia and cognitive deficits
Doklady Biological Sciences
— Regulatory peptides protect brain neurons from hypoxia in vivo
PubMed
— Effects of synthetic peptides in research samples with chronic polymorbidity and organic brain syndrome
Advances in Gerontology (Springer)
— Effects of peptides on behavior and caspase‑3 activity in brain after carotid artery occlusion
Peptide Sciences Research
— Pinealon research: reduction of caspase‑3 and free radical damage
Recess Rx Clinical Reference
— Pinealon: measurement, mechanism, neuroprotection, and clinical considerations
Advances in Gerontology
— Clinical efficacy of peptide bioregulators: comprehensive review and safety profile
PMC ( Drug Measurement Review)
— Pharmacologic considerations of the route
CDC
— Vaccine preparation: route (angle/site; no aspiration)
CDC (Subcut Measurement PDF)
— Technique diagram and site guidance for measurements
NCBI Bookshelf
— Best practices for measurement (asepsis, preparation, and preparation)
