Protocol (2 mL = 5 mg/mL)
| Phase | Example amount (mg) | Units (per measurement) (mL) |
|---|---|---|
| Days 1–2 (Tolerance) | 2.5 mg (2500 mcg) | 50 units (0.50 mL) |
| Days 3+ (Standard) | 5 mg (5000 mcg) | 100 units (1.00 mL) |
| Alternative BID | 2.5 mg twice daily | 50 units (0.50 mL) × 2 |
Frequency: Measure once or twice daily. Due to the compound’s plasma half-life of approximately 3.8–6.9 hours[5], twice-daily (BID) measurement may provide more sustained NNMT inhibition. A single 10 mg vial provides 2–4 days of material at these doses.
Reconstitution Steps
- Remove the vial from freezer storage (−20 °C / −4 °F) and allow to equilibrate at room temperature for 15–20 minutes.
- Draw 2.0 mL bacteriostatic water with a sterile syringe.
- Measure slowly down the vial wall; avoid foaming.
- Gently swirl/roll until dissolved—solution should be clear (do not shake).
- Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light; use within 2–4 weeks.
Important: This guide is for educational purposes only and is not medical advice.
How This Works
5-Amino-1MQ (5-amino-1-methylquinolinium) is a synthetic small molecule that selectively inhibits Nicotinamide N-methyltransferase (NNMT)[1]. NNMT is a cytosolic enzyme that methylates nicotinamide (vitamin B3) using S-adenosylmethionine (SAM) as a methyl donor. In states of obesity and metabolic dysfunction, NNMT is often overexpressed in adipose tissue, depleting nicotinamide and reducing NAD+ availability[2].
By inhibiting NNMT, 5-Amino-1MQ may spare nicotinamide for NAD+ synthesis via the salvage pathway, thereby activating SIRT1 (Sirtuin 1) pathways associated with mitochondrial biogenesis, fat oxidation, and metabolic flexibility[3]. Preclinical studies indicate that NNMT knockdown or inhibition can protect against diet-induced weight gain and improve metabolic parameters without reducing food intake[8][9].