Male Fertility Protocol (3 mL = 25 IU/mL)
| Week/Phase |
Dose per Measurement |
Volume per Measurement |
| Weeks 1–12 |
75 IU (0.15 mg) |
3.0 mL (300 units) |
| Weeks 13–16 (optional extension) |
75 IU (0.15 mg) |
3.0 mL (300 units) |
Frequency: Measure three times per week (for example, Monday, Wednesday, and Friday)[3][4]. HMG research is typically combined with hCG measurements to maximize testosterone production and spermatogenesis[5][6].
Important Volume Note: Each full 75 IU dose requires 3.0 mL, which exceeds standard 1 mL insulin syringe capacity. Options include:
- Use a 3 mL syringe (preferred for single measurement)
- Split dose into three 1 mL measurements at different sites (1 mL = 25 IU each)
- Consider smaller reconstitution volume (1 mL) per manufacturer guidance for reduced measurement volume[8]
Reconstitution Steps
- Draw 3.0 mL bacteriostatic water with a sterile 3 mL syringe.
- Measure slowly down the vial wall to avoid foaming.
- Gently swirl or roll the vial until fully dissolved (do not shake).
- Label with date and time; refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
- For optimal potency, use reconstituted solution promptly or within a few days[9].
Important: This guide is for research educational purposes only and does not constitute medical advice, diagnosis, or research model. For research use only.
How This Works
HMG provides both FSH and LH activity in a 1:1 ratio, derived from purified human menopausal urine[1][2]. In males with hypogonadotropic hypogonadism, FSH stimulation is essential for spermatogenesis (sperm production)[3]. When hCG alone fails to induce adequate sperm production, adding HMG provides the necessary FSH activity to support testicular function and fertility[5][6]. Clinical studies demonstrate that HMG combined with hCG significantly improves sperm parameters including motility, morphology, and concentration, with enhanced pregnancy rates in treated couples[7][12].
References
Metrovan Urology – hMG (Menotropin) Overview
— hMG composition: 1:1 blend of FSH and LH derived from postmenopausal urine; standard preparations provide 75 IU FSH + 75 IU LH per vial
Menopur® (Menotropins) Product Monograph
— Human menopausal gonadotropin for preparation; each vial contains 75 IU FSH + 75 IU LH activity
ASRM Practice Committee 2020 – Gonadotropin Use for Ovulation Induction
— Clinical guidance on gonadotropin research; low-dose protocols (37.5–75 IU) with careful monitoring
ASRM Research sample Fact Sheet – Medications for Inducing Ovulation
— Typical gonadotropin protocols: 75–150 IU measured daily for approximately 7–12 days; course may extend if ovaries respond slowly
Metrovan Urology – Hormones and Male Infertility Research model
— In hypogonadal men, hMG added at 75–150 IU three times weekly if hCG alone insufficient for spermatogenesis
Metrovan Urology – Combined hCG and hMG Research
— hCG given 2–3 times weekly stimulates testosterone; hMG provides FSH activity to stimulate sperm production when FSH deficient
International Journal of Reproductive BioMedicine (Iran JRM, 2014)
— Randomized trial: HMG 75 IU three times weekly for 3 months significantly improved sperm motility and morphology; pregnancy rate 57% vs. control
View Source
Menopur® Monograph – Preparation and Reconstitution
— preparation for follicular stimulation; each 75 IU vial typically reconstituted in 1 mL diluent for 75 IU/mL concentration
Menopur® Monograph – Stability and Storage
— Lyophilized menotropin stable at room temperature (15–25 °C) away from light; reconstituted solution should be used immediately to ensure potency and sterility
MedlinePlus – Measurement Instructions
— Use short, thin needle for SC measurements; insert at 90° angle into pinched fatty tissue (45° if limited fat); do not reuse needles
MedlinePlus – Preparation area Rotation
— Maintain skin health by alternating measurement sites at least one inch apart; avoid areas with irritation, scars, or bruises
Reproductive Medicine and Biology (2018) – Gonadotropin Research for Male Infertility
— Review of hCG and hMG combination research for hypogonadotropic hypogonadism; FSH supplementation critical for spermatogenesis when hCG monotherapy inadequate
