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Research Reference

HCG Research Reference

Complete research reference and reconstitution guide for HCG.

Research Use Only Reconstitution Math Storage Reference

Quick Reference

Vial Amount
5000 IU
CoreVials Standard Mix
Add 1 mL (100 units) BAC Water
Final Concentration
5000 IU/mL
Easy Reference
10 units = 500 IU
Storage
Refrigerate after reconstitution

This page is for research-use-only educational reference and measurement math. It is not medical, veterinary, dosing, administration, or treatment guidance. Products are not for human or animal consumption.

On this page

Standard Protocol (2 mL = 2,500 IU/mL)

Week/Phase Dose per Measurement (IU) Units (per measurement) (mL)
Weeks 1–12 500 IU 20 units (0.20 mL)

Frequency: Measure 3 times weekly (e.g., Monday/Wednesday/Friday). This yields a total weekly dose of 1,500 IU. This schedule maintains intratesticular testosterone during exogenous testosterone research and supports fertility preservation[3][4].

Reconstitution Steps

  1. Draw 2.0 mL bacteriostatic water with a sterile syringe.
  2. Measure slowly down the vial wall; avoid foaming or vigorous shaking.
  3. Gently swirl or roll until the powder fully dissolves (clear solution).
  4. Label vial with reconstitution date and concentration (2,500 IU/mL); refrigerate immediately at 2–8 °C (35.6–46.4 °F).

Measurement Calculations:

  • 250 IU = 10 units (0.10 mL)
  • 500 IU = 20 units (0.20 mL)
  • 1,000 IU = 40 units (0.40 mL)

High‑Dose Protocol (for Post‑Cycle Recovery or Severe Suppression)

Week/Phase Dose per Measurement (IU) Units (per measurement) (mL)
Weeks 1–4 1,500 IU 60 units (0.60 mL)
Weeks 5–8 2,000 IU 80 units (0.80 mL)
Weeks 9–12 1,000 IU 40 units (0.40 mL)

Frequency: Measure 3 times weekly. High‑dose protocols (1,500–2,500 IU per measurement) are used to reactivate testosterone production after prolonged anabolic steroid use or severe hypogonadotropic hypogonadism[5][6]. After initial recovery, doses are typically reduced to maintenance levels (500–1,000 IU 3×/week).

Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

How This Works

HCG is a glycoprotein hormone structurally similar to luteinizing hormone (LH). It binds to the same LH receptors in testicular Leydig cells, stimulating endogenous testosterone production[1]. Unlike endogenous LH (which has a 30‑minute half‑life), HCG’s extended 36‑hour half‑life provides sustained gonadal stimulation[2]. This makes it effective for maintaining testicular function during exogenous testosterone research, where natural LH production is suppressed. Studies show that low‑dose HCG (250–500 IU every other day) maintains intratesticular testosterone at near‑baseline levels in men receiving testosterone research[4]. Higher doses (1,500–5,000 IU multiple times weekly) are used to restore spermatogenesis and endogenous testosterone production in cases of hypogonadotropic hypogonadism or post‑anabolic steroid recovery[5][6].

CoreVials Standard Mix

For a 5000 IU vial Add 1 mL (100 units) BAC Water
Final concentration 5000 IU/mL

After mixing (U-100 syringe reference)

  • 5 units=250 IU
  • 10 units=500 IU
  • 20 units=1000 IU

These are example measurement calculations only. They are not dosing, administration, medical, or veterinary guidance.

Other Mix Options
BAC Added Final Concentration Dose per 10 units
1 mL (100 units) CoreVials Standard Mix 5000 IU/mL 500 IU
2 mL (200 units) 2500 IU/mL 250 IU
1 mL (100 units) 10000 IU/mL 1,000 IU
2 mL (200 units) 5000 IU/mL 500 IU

Supply Planning

Products commonly paired for research preparation:

  • HCG Research Reference
  • BAC Water
  • Cryo labels
  • Storage case

References

National Center for Biotechnology Information (NCBI)
— Human Chorionic Gonadotropin: structure, function, and LH receptor binding mechanisms

Seminars in Reproductive Medicine
— Pharmacokinetics and pharmacodynamics of HCG: extended half‑life and sustained activity

Translational Andrology and Urology (2018)
— Lee JA & Ramasamy R: Review of hCG for hypogonadal male infertility (typical regimens: 1,500–5,000 IU 2–3×/week)

The Journal of Clinical Endocrinology & Metabolism (2005)
— Coviello AD et al: Low‑dose hCG maintains intratesticular testosterone in men with testosterone‑induced gonadotropin suppression

Male Infertility Guide
— Clinical commentary on hCG measurement: 500 IU SC 3×/week for maintenance; 1,000–4,000 IU 3×/week for post‑androgen recovery

Drugs.com (2025)
— HCG Measurement Guide: Adult dose for male hypogonadism: 500–1,000 IU IM 3×/week for 3 weeks, then 2×/week; or 4,000 IU 3×/week for 6–9 months

Mayo Clinic
— Chorionic Gonadotropin drug description: uses for ovulation induction, sperm production, and cryptorchidism research model

University Hospitals Fertility Center
— Research sample instructions for low‑dose HCG: storage at room temperature (unreconstituted); refrigeration after mixing

FDA Prescribing Information
— Chorionic Gonadotropin (Pregnyl/Novarel): reconstituted solution stable for 60 days when refrigerated; do not freeze

University Hospitals Fertility Center
— SC handling reference for HCG: clean site, pinch skin, insert straight in, measure slowly, wait before removing needle; never reuse syringes

Healthline (2019)
— How to Measure hCG: step‑by‑step SC and IM measurement instructions for fertility and hormone research applications

Centers for Disease Control and Prevention (CDC)
— Vaccine preparation guidelines: handling reference (45–90° angle, no aspiration required)

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NCBI Bookshelf
— Best practices for medication measurement: aseptic technique, preparation, and safe preparation procedures

British Journal of Clinical Pharmacology (1995)
— Lijesen GK et al: Meta‑analysis concluding no scientific evidence supports HCG for weight loss (obesity diet context)

Research Use Notice

This page is research-use-only educational reference and measurement math. It is not medical, veterinary, dosing, administration, or treatment guidance. Products are not for human or animal consumption.

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